Table 5 Safety test of FC-S spraying on laying hens

Treatments	After 1^stspraying				After 2^ndspraying
Treatments	Immediatelybefore spraying	Immediatelyafter spraying	After 4 h	After 1∼7 d	Immediatelybefore spraying	Immediatelyafter spraying	After 4 h	After 1∼14 d
NC	-	-	-	-	-	-	-	-
PC	-	-	-	-	-	-	-	-
FC-S 0	-	-	-	-	-	-	-	-
FC-S 50	-	-	-	1 death (4 d)	-	-	-	-
FC-S 100	-	-	-	-	-	-	-	-

Safety test confirmed abnormal symptoms (abnormal feed intake and respiration, lethargy, diarrhea and mortality). FC-S (Farm Clean-S) included Chamaecyparis obtusa essential oil at 70%; NC (negative control): distilled water; PC (positive control): commercial spray solution for eliminating red mite; FC-S 0: FC-S undiluted; FC-S 50: FC-S diluted with 50 times distilled water; FC-S 100: FC-S diluted with 100 times distilled water.