Table 5. Comparison of the expression level of HSP-70 gene of hatched chickens in summer and winter

Hatched	10 wks		20 wks		40 wks
	ΔCt	2−ΔΔCt	ΔCt	2−ΔΔCt	ΔCt	2−ΔΔCt
Summer	0.82±0.45*	1	−0.06±1.46*	1	−0.76±0.76**	1
Winter	−0.57±1.23	2.63	−0.98±1.11	1.94	−2.01±0.93	2.45

Values are means±standard deviation.
ΔCt value which is equal to the difference in threshold cycles for target and internal control gene.
The values are 2^−ΔΔct which indicates the fold change in gene expression relative to the control.
Values in the same column are significantly different (^* P<0.05, ^** P<0.01).