Table 2. Sequence of S1 gene specific primers

Target gene	Primer	Sequence (5’-3’)	PCR product size
S1 gene	IBV F1	AGCAACRCCAGTTGTDAATTTG	700~800 bp
	IBV R1	CWGTACCATTAACAAARTAAGCMAG
	IBV F2	TGTGTATTTTAAAGCAGGTGGACC
	IBV R2	GTTTGTATGTACTCATCTGTAAC	1,000~1,100 bp