Table 2. PCR primers for detection of poultry pathogens in the study

Pathogen	Target gene	Sequence(5--- 3’)	Band size
Mycoplasma gallisepticum	Mgc2	F: TGG GAT TCC GAT CGC TAA GAA	545 bp
		R: TAA ACC CAC CTC CAG CTT TAT TTC C
Mycoplasma synoviae	vlhA	F: GGC CAT TGC TCC TRC TGT TAT	370 bp
		R: AGT AAC CGA TCC GCT TAA TGC
H9N2 influenza	M gene	F: AGA TGA GTC TTC TAA CCG AGG TCG	55 bp
		R: TGC AAA AAC ATC TTC AAG TCT CTG
		Probe: FAM-TCA GGC CCC CTC AAA GCC GA-TAMRA