Table 7. Effects of breed, laying age and egg storage period on the HSP gene expression levels and the H/L ratio in hatched chicks

Factors HSP-70 HSP-90α HSP-90β H/L ratio
ΔCT 2−ΔΔct ΔCT −ΔΔct ΔCT 2−ΔΔct
Breed (A) Jaeraejong -1.8±2.6ab 1 25.8±4.6 1 5.2±1.7 1 0.15±0.16b
K. Rhode -1.14±3.9a 0.65 25.8±4.7 1 5.8±1.7 0.68 0.17±0.16ab
K. Leghorn -2.09±2.6b 1.26 24.8±4.8 2 5.1±4.3 1.12 0.20±0.16a
Laying age (B) 27~29wk -1. 1±2.2a 1 26.5±5.8a 1 5.7±3.6a 1 0.14±0.12b
50 ~ 52wk -2.2±1.8b 2.1 24.3±4.0b 4.59 5.0±1.5b 1.62 0.20±0.18a
Egg storage period(C) 3d -1.2±4.0 1 25.5±4.4 1 5.4±1.9 1 0.19±0.19
7d -2.0±2.4 1.74 25.5±4.8 1 5.3±4.0 1.07 0.16±0.14
14d -1.9±2.8 1.64 25.1±5.0 1.32 5.3±1.7 1.07 0.16±0.12
P values A 0.0394 0.2485 0.1362 0.0396
B <0001 <0001 0.0222 0.0017
C 0.4501 0.7725 0.9888 0.1895
A × B 0.7051 0.5503 0.3009 0.2273
A × C 0.0071 0.5238 0.1107 0.0286
B × C 0.0007 0.1857 0.7983 0.8829
A × B × C 0.2376 0.0022 0.2880 0.7950
Values are mean±standard deviation.
The different superscripts within column in each factor significantly differ.