Table 1.
Primers used for the detection of outer membrane and LPS synthesis-associated genes in R. anatipestifer

Genes	Location	Description of genes	Primer sequence (5’-3’)	Size (bp)	Annealing temperature	Accession no.	Source
AS87_09335	Outer membrane	Membrane protein	F: GGACTGCTTTACCTTTGTCTG	1174	60°C	NZ_CP007204.1	This study
			R: ATACTGGTCTTTGGCACGC
AS87_05195	Outer membrane	Hypothetical protein	F: ATTGACATTATGCGTCGTGG	774	55°C	NZ_CP007204.1	This study
			R: TGCCGTCTGAACCTTGAAA
AS87_00480	Outer membrane	Hypothetical protein	F: ACCACAAAGGTAACCCAAGA	1180	60°C	NZ_CP007204.1	This study
			R: TGTCCTGAAGTCTCCGCTA
AS87_04050	Cytoplasmic membrane	Vi polysaccharide biosynthesis protein VipB/TviC	F: CCGCTCGAGATGAATAACAGGAAAATATTAATTACTGGAGGGGCAGG	972	65°C	NZ_CP007204.1	Wang et al., 2014
			R: CATGCATGCTTATTTCAACCTCTTCCAATACCAATCCAC
AS87_01015	Cytoplasmic membrane	Glycosyl transferase family 2	F: AATCTTCATTTCACCAGCCG	328	55°C	NZ_CP007204.1	This study
			R: TTATCCCAACCCAATACGGA
OmpA	Outer membrane	Outer membrane protein	F: ATGTTGATGACTGGACTTGGTCT	1119	54°C	AF104936.1	Yu et al., 2008
			R: CTTCACTACTGGAAGGTCAGACTT