| Primer name | Primer sequence (5′ to 3′) | Tm (°C) | Product size (bp) |
|---|---|---|---|
| TRIM63-F | GAT TTT TGG GGT GCA GAG AA | 58 | 201 |
| TRIM63-R | CTG GGG AGT CAG GAT GAA AA | - | - |
| FBXO32-F | GGC TGC TGT GGA AGA AAC TC | 58 | 188 |
| FBXO32-R | CCA AGA GAG GAT GTG GCA AT | - | - |
| SMAD2-F | CAG AAA CGC AAC AGT GGA GA | 58 | 174 |
| SMAD2-R | GGT GGG ATT TTG CAG ACA GT | - | - |
| SMAD3-F | TGG CTC AGT CTG TGA ACC AG | 60 | 190 |
| SMAD3-R | CCC ATC TGT GTG AGG ACC TT | - | - |
| FOXO1-F | AAG CCC CAG CTC TCA CAG TA | 60 | 203 |
| FOXO1-R | TCT CTG AAA GGC TGG GAA GA | - | - |
| GAPDH-F | GGA CAC TTC AAG GGC ACT GT | 60 | 160 |
| GAPDH-R | TCT CCA TGG TGG TGA AGA CA | - | - |