Table 7. Effects of breed, laying age and egg storage period on the HSP gene expression levels and the H/L ratio in hatched chicks
Factors | | HSP-70 | HSP-90α | HSP-90β | H/L ratio |
ΔCT | 2−ΔΔct | ΔCT | −ΔΔct | ΔCT | 2−ΔΔct |
Breed (A) | Jaeraejong | -1.8±2.6ab | 1 | 25.8±4.6 | 1 | 5.2±1.7 | 1 | 0.15±0.16b |
K. Rhode | -1.14±3.9a | 0.65 | 25.8±4.7 | 1 | 5.8±1.7 | 0.68 | 0.17±0.16ab |
K. Leghorn | -2.09±2.6b | 1.26 | 24.8±4.8 | 2 | 5.1±4.3 | 1.12 | 0.20±0.16a |
Laying age (B) | 27~29wk | -1. 1±2.2a | 1 | 26.5±5.8a | 1 | 5.7±3.6a | 1 | 0.14±0.12b |
50 ~ 52wk | -2.2±1.8b | 2.1 | 24.3±4.0b | 4.59 | 5.0±1.5b | 1.62 | 0.20±0.18a |
Egg storage period(C) | 3d | -1.2±4.0 | 1 | 25.5±4.4 | 1 | 5.4±1.9 | 1 | 0.19±0.19 |
7d | -2.0±2.4 | 1.74 | 25.5±4.8 | 1 | 5.3±4.0 | 1.07 | 0.16±0.14 |
14d | -1.9±2.8 | 1.64 | 25.1±5.0 | 1.32 | 5.3±1.7 | 1.07 | 0.16±0.12 |
P values | A | 0.0394 | | 0.2485 | | 0.1362 | | 0.0396 |
B | <0001 | | <0001 | | 0.0222 | | 0.0017 |
C | 0.4501 | | 0.7725 | | 0.9888 | | 0.1895 |
A × B | 0.7051 | | 0.5503 | | 0.3009 | | 0.2273 |
A × C | 0.0071 | | 0.5238 | | 0.1107 | | 0.0286 |
B × C | 0.0007 | | 0.1857 | | 0.7983 | | 0.8829 |
A × B × C | 0.2376 | | 0.0022 | | 0.2880 | | 0.7950 |
Values are mean±standard deviation.
The different superscripts within column in each factor significantly differ.